Regulation and glutamic acid decarboxylase during Neurospora crassa conidial germination

Polyamine regulation of ornithine decarboxylase synthesis in Neurospora crassa.

Ornithine decarboxylase (ODC) of the fungus Neurospora crassa, encoded by the spe-1 gene, catalyzes an initial and rate-limiting step in polyamine biosynthesis and is highly regulated by polyamines. In N. crassa, polyamines repress the synthesis and increase the degradation of ODC protein. Changes in the rate of ODC synthesis correlate with similar changes in the abundance of spe-1 mRNA. We ide...

Deacetylation of N-acetyl-L-glutamic acid by Neurospora crassa.

Photosynthetic bacteria and blue-green algae synthesize ornithine from glutamic acid by a cyclic route in which the intermediate N-acetylglutamic acid is regenerated by a transacetylation reaction; furthermore, in these microorganisms, arginine, which is synthesized from ornithine, is a specific inhibitor of N-acetyl-,y-glutamokinase which phosphorylates N-acetylglutamic acid on its y-carboxyl ...

The Guanine Nucleotide Exchange Factor RIC8 Regulates Conidial Germination through Gα Proteins in Neurospora crassa

Heterotrimeric G protein signaling is essential for normal hyphal growth in the filamentous fungus Neurospora crassa. We have previously demonstrated that the non-receptor guanine nucleotide exchange factor RIC8 acts upstream of the Gα proteins GNA-1 and GNA-3 to regulate hyphal extension. Here we demonstrate that regulation of hyphal extension results at least in part, from an important role i...

Neurospora crassa Hyphal Fusion in Pathway during Conidial Germination and Role of a Mitogen-Activated Protein Kinase

Polysomes, ribonucleic acid, and protein synthesis during germination of Neurospora crassa conidia.

The level of polysomes in ungerminated conidia of Neurospora crassa depends on the method used to collect spores. Spores harvested and exposed to hydration contain 30% of their ribosomes as polysomes, whereas those not exposed to hydration contain only 3% of their ribosomes as polysomes. During the germination process, the percentage of the ribosomes which sediment as polysomes increases rapidl...